Journal: Current protocols in microbiology
Article Title: Construction of a Transcription Map for Papillomaviruses using RACE, RNAse Protection and Primer Extension Assays
doi: 10.1002/9780471729259.mc14b06s40
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Article Snippet: Total RNAs (from step 9, Basic Protocol 1) list-behavior=simple prefix-word= mark-type=none max-label-size=0 RQ1 RNase-Free DNase 10× Reaction Buffer (400 mM Tris-HCl, pH 8.0, 100 mM MgSO 4 , 10 mM CaCl 2 (Promega) RQ1 RNase-Free DNase (Promega) RQ1 DNase Stop Solution (20 mM EGTA, pH 8.0, Promega) Nuclease-free water DEPC-Treated Water Following reagents from Life Technologies list-behavior=simple prefix-word= mark-type=none max-label-size=0 10× PCR Buffer II (200 mM Tris-HCl,pH 8.4, 500 mM KCl) MgCl 2 (25 mM) dNTP Mix (10 mM each) Taq DNA polymerase (5 U/μl) RNase Inhibitor (40 U/μl) Random Hexamer (50 μM) MuLV Reverse Transcriptase (50 U/μl) Primer design list-behavior=simple prefix-word= mark-type=none max-label-size=2 1.
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